Mouse Anti-PlGF Recombinant Antibody (clone AB047)
CAT#: HPAB-0723-FY
This product is a recombinant antibody that recognizes PlGF. The monoclonal antibody AB047 reacts with the epitope of PlGF.
Tested Data
WB
Figure 1 Anti-PIGF Recombinant Antibody (HPAB-0723-FY) in WB
Western blot analysis of HPAB-0723-FY was performed by loading human PIGF protein in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0723-FY (2 μg/mL) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 5000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-PIGF Recombinant Antibody (HPAB-0723-FY) in DB
Dot Blot analysis of HPAB-0723-FY was performed by coating with human PIGF protein.
HPAB-0723-FY incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
ELISA
Figure 3 Anti-PIGF Recombinant Antibody (HPAB-0723-FY) in ELISA
ELISA analysis of HPAB-0723-FY was performed by coating with human PIGF protein. Then blocked with BSA and incubated with HPAB-0723-FY. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PlGF
- Species Reactivity
- Human
- Clone
- AB047
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Placental growth factor; PlGF; PGF
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0723-FY, RRID: AB_3111406)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone AB047"
- CAT
- Product Name
See other products for "PlGF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0723-FY-F(E) | Mouse Anti-PlGF Recombinant Antibody (clone AB047); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0723-FY-S(P) | Mouse Anti-PlGF Recombinant Antibody (clone AB047); Fab Fragment | ELISA | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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